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Full Description
Techniques in molecular biology continue to develop and find wider applications both in research and the biotechnology industry. DNA Cloning aims to extend and complement existing cloning manuals. Contents of Volume I range from the use of phage lamda replacement vectors in the construction of representative genomic DNA libraries to broad host range cloning vectors for Gram-negative bacteria.
Contents
The use of phage lambda replacement vectors in the construction of representative genomic DNA libraries; Constructing and screening cDNA libraries in gt10 and gt11; An alternative procedure for the synthesis of double-stranded cDNA for cloning in phage and plasmid vectors; Immunological detection of chimeric B-galactosidases expressed by plasmid vectors; The pEMBL family of single-stranded vectors; Techniques for transformation of E.Coli; The use of genetic markers for the selection and allelic exchange of in vitro induced mutations that do not have a phenotype in E. Coli; The oligonucleotide-directed construction of mutations in recombinant filamentous phage; Broad host range cloning vectors for gram negative bacteria
